Signalling

Part:BBa_K872000:Design

Designed by: Ricardo Munguía Díaz   Group: iGEM12_Ciencias_UNAM   (2012-09-26)

Signal converter, converts a HCO3- signal in a cAMP intermediate signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 898
    Illegal XhoI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 525
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is very sensitive, low concentrations of HCO3- activetes the cAMP signalitation. An BamHI restriction site is added.

Source

Promoter J23101 secuence can be found in https://parts.igem.org/Part:BBa_J23101:Design Rbs B0012 secuence can be found in https://parts.igem.org/Part:BBa_B0012:Design sAC Protein secuence can be found in http://www.genome.jp/dbget-bin/www_bget?bmy:Bm1_53800 Terminator B0030 can be found in https://parts.igem.org/Part:BBa_B0030:Design

References