Part:BBa_K872000:Design

Signal converter, converts a HCO3- signal in a cAMP intermediate signal

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 714
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 898
  Illegal XhoI site found at 304
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 525
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is very sensitive, low concentrations of HCO3- activetes the cAMP signalitation. An BamHI restriction site is added.

Source

Promoter J23101 secuence can be found in https://parts.igem.org/Part:BBa_J23101:Design Rbs B0012 secuence can be found in https://parts.igem.org/Part:BBa_B0012:Design sAC Protein secuence can be found in http://www.genome.jp/dbget-bin/www_bget?bmy:Bm1_53800 Terminator B0030 can be found in https://parts.igem.org/Part:BBa_B0030:Design